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  5. Immigration DNA Tests
  6. Cell Line Authentication
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Human Cell Line Authentication and Mycoplasma Detection

 


  • Pricing

  • Order and Shipping

  • Using DNA Collection Cards

  • Compare & Interpret your results

  • About DDC

Cell lines are often used in biomedical research to investigate disease pathways and therapies.

In late 2007, an open letter by a group of scientists led by Dr. Roland Nardone spurred the National Institutes of Health (NIH) to recommend authenticating cell lines in scientific studies.

In early 2012, the ATCC issued its standard to address the problem of cell line misidentification and "delineate a standardized, universally applicable method for authenticating new and established human cell lines and human primary tissue used in research."

Studies have shown an alarming percentage of human cell lines, those purchased from commercial repositories and primary cell lines, are contaminated or misidentified. Poor cell culture technique, shared equipment and poor quality control in a laboratory can all contribute to the misidentification and contamination issues. In addition, genetic instability of some cell lines can result in changes at the DNA level that can compromise research results. Articles published on these topics indicate as much as 20 to 33 percent of all cell lines are misidentified or contaminated, but there is a simple and affordable solution.

DDC Medical, a division of DNA Diagnostics Center (DDC), one of the world’s largest DNA laboratories, offers Human Cell Line Authentication and Mycoplasma Detection services.

Peer review Journals throughout the research community recommends Cell Line Authentication and Mycoplasma Detection as part of the publication process. Grant providers are also aware of the issue and requiring Cell Line Authentication and Mycoplasma Detection ensures a sound investment.

DDC Medical Customers

   • Academia Researchers and Teaching Labs
   • Pharma Labs and Researchers
   • Biotech Labs and Researchers
   • Government Labs and Researchers
   • Other University, private and non-profit cancer and cell-based research institutions

When to Conduct Cell Line Authentication

   • When a new line is first established or purchased
   • As a control measure at the start of a new study and concluding a study
   • At least once every 6 months in active culture
   • Before freezing down for storage
   • When an unexpected result occurs



Human Cell Line and Primary Cell Line Authentication

DDC Medical is committed to assisting the research community with proper cell line documentation through its Cell Line Authentication and Mycoplasma Detection services. We offer the most comprehensive testing available. DDC Medical uses DNA STR analysis, examining 17 highly polymorphic genetic markers, more than twice than is recommended. A DNA profile is generated, uniquely identifying the cell line. Authentication will ensure the research has been conducted using the correct cells, maximizing reliable results, supporting publication efforts and reducing the risk of wasted time and resources. A researchers reputation could also be adversely affected by publishing results from contaminated cell lines.





DDC uses STR (Short Tandem Repeat) Analysis in Cell Line Authentication

The DNA profile generated for Cell Line Authentication utilizes STR analysis of a number of highly polymorphic unlinked loci. Each locus is characterized by a motif of repeat units typically 4 bases in length. The number of repeats detected in a DNA fragment identifies each allele. For example, if the designation at the D5S818 locus is 14, 16, two DNA fragments were detected; one with 14 repeats and one with 16 repeats. The loci used for Cell Line Authentication are utilized for human identification for paternity and forensic analysis and are not designed to detect non-human DNA. Cell lines established from different human sources will possess different DNA profiles.


Mycoplasma Detection

Authenticating cell cultures is a process that not only verifies the identity of the cell line, but also rules out any contaminants. Mycoplasma is one of the most prevalent contaminating agents in cell culture. Unlike most bacterial and fungal contaminants, which are easily detected by changes in visual appearance of the culture, mycoplasma contamination cannot be detected without further analysis.

Mycoplasma are parasitic, unicellular organisms that have the ability to induce chromosomal aberrations and metabolic changes, as well as alter a cell line's growth pattern, in some cases leading to the death of the cell line. These changes can affect the experimental results derived from infected cell lines.

DDC’s Mycoplasma Detection Test

To rule out mycoplasma in your cell cultures, DDC Medical offers a DNA PCR detection test that will screen for 19 species, including the 6 most common contaminants found in cell cultures. DDC Medical tests for all of the most common contaminants and 95% of all mycoplasma contaminants. The Mycoplasma Detection test has a sensitivity of 3 to 5 femtograms of mycoplasma DNA or approximately 5 mycoplasma genomes per micro liter.

A. laidlawii (++)

M. arginini (++)

M. arthritidis

M. bovis

M. cloacale

M. falconis

M. agalactiae

M. faucium (++)

M. fermentans (++)

M. hominis

M. hyorhinis (++)

M. hyosynoviae

M. opalescens

M. orale (++)

M. pulmonis

M. spermatophilum

M. salivarium

M. primatum

M. timone



Ordering Information

Please note that if you are ordering both Human Cell Line Authentication and Mycoplasma Detection, you only need to provide one sample for both tests.

Call 01 506 0395 To Order!

If you are interested in becoming a distributor, please email us: dleigh@dna-bioscience.co.uk


Cell Line Authentication


Cell Line Authentication

Mycoplasma Detection

Pricing

Samples (1-9): € 122 each

Samples (10+): € 114 each
Mouse Cell Line Samples: €192 each

Samples (1-9): €60 each

Samples (10+): €53 each

 

We will require one of the following:
  • • Purified DNA - 10-20 μl at a minimum of 1 ng/μl

  • • Cell pellet containing 1x105 cells

  • • FTA or cell collection card at concentration of 1x106 ml (up to 100 ul)
  • • Purified DNA - 10-20 μl at a minimum of 1 ng/μl

  • • Cell pellet containing 1x105 cells

  • • FTA or cell collection card at concentration of 1x106 / ml (up to 100 ul)

  • • Cell culture supernatant 100μl
What DDC will provide You with:
  • • Electronic notarised certificate of analysis that includes a DNA profile of 17 highly polymorphic genetic markers plus amelogenin to determine gender

  • • Confirm the presence of small amounts of mouse DNA in a human cell culture due to contamination from feeder cells

  • • Electropherogram (€19 extra)

  • • 3-5 day turnaround

  • • 1-2 day turnaround (cost dependent on order size)

  • Interpret your results. (ATCC, DSMZ, COG, JCRB)
  • • Electronic notarised certificate of analysis indicating presence or absence of 19 species of mycoplasma..

  • • 3-5 day turnaround

  • • 1-2 day turnaround (cost dependent on order size)

  • Interpret your results. (ATCC, DSMZ, COG, JCRB)

Order Form and Shipping Instructions

  1. Download a PDF of the order form here or call DDC at 01 506 0395 to get started.
  2. Complete order form and fax to +44 (0)20 8563 1858
  3. Carefully follow shipping instructions.

Sample Shipping Instructions

View shipping instructions here. DNA collection card shipping instructions are also included in the shipping form.



References

Below are recent articles discussing the development of cell line authentication guidelines.

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