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Cell lines are often used in biomedical research to
investigate disease pathways and therapies.
In late 2007, an open letter by a group of scientists led by Dr. Roland
Nardone spurred the National Institutes of Health (NIH) to recommend
authenticating cell lines in scientific studies.
In early 2012, the ATCC issued its standard to address the problem
of cell line misidentification and "delineate a standardized, universally
applicable method for authenticating new and established human cell
lines and human primary tissue used in research."
Studies have shown an alarming percentage
of human cell lines, those purchased from commercial
repositories and primary cell lines, are contaminated
or misidentified. Poor cell culture technique, shared
equipment and poor quality control in a laboratory
can all contribute to the misidentification and contamination
issues. In addition, genetic instability of some
cell lines can result in changes at the DNA level
that can compromise research results. Articles published
on these topics indicate as much as 20 to 33 percent
of all cell lines are misidentified or contaminated,
but there is a simple and affordable solution.
DDC Medical, a division of DNA Diagnostics
Center (DDC), one of the world’s largest DNA laboratories,
offers Human Cell Line Authentication and Mycoplasma
Peer review Journals throughout the
research community recommends Cell Line Authentication
and Mycoplasma Detection as part of the publication
process. Grant providers are also aware of the issue
and requiring Cell Line Authentication and Mycoplasma
Detection ensures a sound investment.
DDC Medical Customers
Researchers and Teaching Labs
• Pharma Labs and Researchers
• Biotech Labs and Researchers
• Government Labs and
• Other University, private
and non-profit cancer and cell-based research institutions
When to Conduct Cell Line Authentication
a new line is first established or purchased
• As a control measure
at the start of a new study and concluding a study
• At least once every
6 months in active culture
• Before freezing down
• When an unexpected
Cell Line and Primary Cell Line Authentication
is committed to assisting the research community with
proper cell line documentation through its Cell Line
Authentication and Mycoplasma Detection services. We
offer the most comprehensive testing available. DDC
Medical uses DNA STR analysis, examining 17 highly
polymorphic genetic markers, more than twice than is
recommended. A DNA profile is generated, uniquely identifying
the cell line. Authentication will ensure the research
has been conducted using the correct cells, maximizing
reliable results, supporting publication efforts and
reducing the risk of wasted time and resources. A researchers
reputation could also be adversely affected by publishing
results from contaminated cell lines.
STR (Short Tandem Repeat) Analysis in Cell Line Authentication
The DNA profile generated for
Cell Line Authentication utilizes STR analysis of a number of highly
polymorphic unlinked loci. Each locus is characterized by a motif of
repeat units typically 4 bases in length. The number of repeats detected
in a DNA fragment identifies each allele. For example, if the designation
at the D5S818 locus is 14, 16, two DNA fragments were detected; one
with 14 repeats and one with 16 repeats. The loci used for Cell Line
Authentication are utilized for human identification for paternity and
forensic analysis and are not designed to detect non-human DNA. Cell
lines established from different human sources will possess different
Authenticating cell cultures is a process that not only
verifies the identity of the cell line, but also rules out any contaminants.
Mycoplasma is one of the most prevalent contaminating agents in cell
culture. Unlike most bacterial and fungal contaminants, which are easily
detected by changes in visual appearance of the culture, mycoplasma
contamination cannot be detected without further analysis.
Mycoplasma are parasitic, unicellular organisms that have
the ability to induce chromosomal aberrations and metabolic changes,
as well as alter a cell line's growth pattern, in some cases leading
to the death of the cell line. These changes can affect the experimental
results derived from infected cell lines.
DDC’s Mycoplasma Detection Test
To rule out mycoplasma in your
cell cultures, DDC Medical offers a DNA PCR detection test that will
screen for 19 species, including the 6 most common contaminants
found in cell cultures. DDC Medical tests for all of the most common
contaminants and 95% of all mycoplasma contaminants. The Mycoplasma
Detection test has a sensitivity of 3 to 5 femtograms of mycoplasma
DNA or approximately 5 mycoplasma genomes per micro liter.
Please note that if you are ordering both Human Cell Line
Authentication and Mycoplasma Detection, you only need to provide one
sample for both tests.